Seems to be a hard work, there are to find a lot of questions concerning this problem but a few answers only.Here you find " A brief note on jatropha tissue culture work based on experiments conducted during past 2 yrs are as follows "http://www.protocol-online.org/biology-forums/posts/3575.html in the bottom third of site.Here a list of publications unfortunately without links:http://www.newcrops.uq.edu.au/listing/jatrophacurcas.htm"In Jatropha curcas shoot bud proliferation using nodal segments as explants was achieved on MS medium supplemented with 2 mg/ l BAP plus 3 mg/ l Kinetin"( page 44 of http://www.tnau.ac.in/ar/an200304/Chapter4.pdfShort article:http://www.scisoc.or.th/stt/32/sec_f/paper/stt32_F_F0007.pdfHope it helps a bit!